Catalog No: OKCD03537
Size:96WELLS
Price: $881.00
SKU
OKCD03537
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CSF2 Chemi-Luminescent ELISA Kit (Dog) (OKCD03537)

Datasheets/ManualsPrintable datasheet for CSF2 Chemi-Luminescent ELISA Kit (Dog) (OKCD03537)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)97-104%79-102%84-93%82-103%
EDTA Plasma (n=5)92-103%94-103%94-101%84-99%
Heparin Plasma (n=5)99-105%80-99%79-104%79-96%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9083
EDTA Plasma (n=5)91-10397
Heparin Plasma (n=5)79-9683
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Csf2 Microplate96 Wells (12 x 8 Well strips)
Csf2 Lyophilized Standard2
100X Csf2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCSF2
Gene Full Namecolony stimulating factor 2
Alias Symbolscolony-stimulating factor;CSF;GM-CSF;granulocyte-macrophage colony-stimulating factor.
NCBI Gene Id403923
Protein NameGranulocyte-macrophage colony-stimulating factor
Description of TargetCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Uniprot IDP48749
Protein Accession #NP_001003245
Nucleotide Accession #NM_001003245
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