CS ELISA Kit (Mouse) (OKCD02464)

Catalog No: OKCD02464

Size:96WELLS

Mouse CS ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Citrate Synthase (CS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Citrate Synthase (CS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Citrate Synthase (CS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Citrate Synthase (CS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Citrate Synthase (CS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Citrate Synthase (CS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CS Microplate: 96 Wells (12 x 8 Well strips)
  • CS Lyophilized Standard: 2
  • 100X Biotinylated CS Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.63 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Citrate Synthase (CS). No significant cross-reactivity or interference between Citrate Synthase (CS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cs
• Gene Full Name: citrate synthase
• Alias Symbols: 2610511A05Rik;9030605P22Rik;ah;Ahl4;BB234005;C;Cis;citrate (Si)-synthase;citrate synthase, mitochondrial.
• NCBI Gene Id: 12974
• Protein Name: Citrate synthase, mitochondrial
• Description of Target: Citrate synthase, mitochondrial
• Uniprot ID: Q9CZU6
• Protein Accession #: NP_080720.1
• Nucleotide Accession #: NM_026444.4
• Protein Size (# AA): 464