CRYM ELISA Kit (Bovine) (OKEH07762)

Catalog No: OKEH07762

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CRYM ELISA Kit (Bovine) (OKEH07762) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRYM has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRYM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRYM captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.0% (n = 20)
  Mean Inter-assay CV%: <=4.4% (n = 20)
• ELISA Kit Component:
  • CRYM Microplate: 96 Wells (12 x 8 Well strips)
  • CRYM Lyophilized Standard: 2
  • 100X Biotinylated CRYM Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.
  Subcellular Location: Cytoplasm
  Unigene: Bt.2913
  SMR: Q2KHX6
  String: 9913.ENSBTAP00000012972
  KEGG: bta:505167
  Pfam: PF02423
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Ketimine reductase mu-crystallin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CRYM
• Gene Full Name: crystallin mu
• Alias Symbols: ketimine reductase mu-crystallin;mu-crystallin homolog;NADP-regulated thyroid-hormone-binding protein;thiomorpholine-carboxylate dehydrogenase.
• NCBI Gene Id: 505167
• Protein Name: Q2KHX6
• Description of Target: Ketimine reductase mu-crystallin
• Uniprot ID: https://www.uniprot.org/uniprot/Q2KHX6
• Protein Accession #: NP_001039379.1
• Nucleotide Accession #: NM_001045914.2