Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH06497
Size:96T
Price: $525.00
SKU
OKEH06497
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Crygd ELISA Kit (Mouse) (OKEH06497) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Crygd antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Crygd and incubated. The Crygd found in the sample or standards competes with the biotinylated Crygd for limited binding sites on the immobilized anti-Crygd antibody. Excess unbound biotinylated Crygd and sample or standard Crygd is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Crygd captured in the well and inversely proportional to the amount of Crygd which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 105.9%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 8.3% (n = 20)
Mean Inter-Assay Precision - <= 10.1% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.426 ng/mL
SpecificityNatural and recombinant Mouse Gamma-crystallin D
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCrygd
Gene Full Namecrystallin, gamma D
Alias SymbolsAey;Aey4;Cryg-;Cryg-1;DGcry-;DGcry-1;gamma-crystallin 1;gamma-crystallin D;gamma-D-crystallin;lens opacity 12;Lop12.
NCBI Gene Id12967
Protein NameP04342
Description of TargetGamma-crystallin D
Uniprot IDhttps://www.uniprot.org/uniprot/P04342
Protein Accession #NP_001077372
Nucleotide Accession #NM_001083903
Write Your Own Review
You're reviewing:CRYGD ELISA Kit (Mouse) (OKEH06497)
Your Rating
We found other products you might like!