CRYBB2 ELISA Kit (Mouse) (OKCD00968)

Catalog No: OKCD00968 (Formerly GWB-KBBNJ7)

Size:96WELLS

Mouse Crybb2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	79-90%	93-101%	87-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Beta B2 (CRYbB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crystallin Beta B2 (CRYbB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Beta B2 (CRYbB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Crystallin Beta B2 (CRYbB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	88-99	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Crystallin Beta B2 (CRYbB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Crystallin Beta B2 (CRYbB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Crybb2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Crybb2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Crybb2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Crystallin Beta B2 (CRYbB2). No significant cross-reactivity or interference between Crystallin Beta B2 (CRYbB2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Crybb2
• Gene Full Name: crystallin, beta B2
• Alias Symbols: Aey;Beta-B2 crystallin;beta-crystallin B2;beta-crystallin Bp;Cryb-;Cryb-2.
• NCBI Gene Id: 12961
• Protein Name: Beta-crystallin B2
• Description of Target: Crystallins are the dominant structural components of the vertebrate eye lens.
• Uniprot ID: P62696
• Protein Accession #: NP_031799
• Nucleotide Accession #: NM_007773.4
• Protein Size (# AA): 205