CRYAB ELISA Kit (Mouse) (OKEH04153)

Catalog No: OKEH04153

Size:96T

Mouse CRYAB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Cryab ELISA Kit (Mouse) (OKEH04153) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cryab has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cryab is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cryab captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 7.5% (n = 20)
  Mean Inter-assay CV%: <= 10.0% (n = 20)
• ELISA Kit Component:
  • Anti-Cryab Microplate: 96 Wells (12 x 8 Well Strips)
  • Cryab Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Cryab Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Mouse Alpha-crystallin B chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cryab
• Gene Full Name: crystallin, alpha B
• Alias Symbols: alpha(B)-crystallin;alpha-crystallin B chain;Cry;Crya;Crya2;Crya-2;crystallin, alpha 2;heat shock protein CryAB;heat shock protein HspB5;Hsp;HspB5;P23.
• NCBI Gene Id: 12955
• Protein Name: P23927
• Description of Target: Alpha-crystallin B chain
• Uniprot ID: https://www.uniprot.org/uniprot/P23927
• Protein Accession #: NP_001276711.1
• Nucleotide Accession #: NM_001289782.1
• Protein Size (# AA): 175
• Molecular Weight: 20 kDa