CRYAB ELISA Kit (Human) (OKCD08324)

Catalog No: OKCD08324

Size:96WELLS

CRYAB ELISA Kit (Human) (OKCD08324) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Alpha B (CRYaB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crystallin Alpha B (CRYaB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Alpha B (CRYaB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Crystallin Alpha B (CRYaB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Crystallin Alpha B (CRYaB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Crystallin Alpha B (CRYaB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CRYAB Microplate: 96 Wells (12 x 8 Well strips)
  • CRYAB Lyophilized Standard: 2
  • 100X CRYAB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.239ng/mL

Target Info

• Gene Symbol: CRYAB
• Gene Full Name: crystallin alpha B
• Alias Symbols: Alpha(B)-crystallin;alpha-crystallin B chain;CMD1II;CRYA2;CTPP2;CTRCT16;epididymis secretory protein Li 101;heat shock protein beta-5;heat-shock 20 kD like-protein;HEL-S-101;HSPB5;MFM2;renal carcinoma antigen NY-REN-27;rosenthal fiber component.
• NCBI Gene Id: 1410
• Protein Name: Alpha-crystallin B chain
• Description of Target: May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
• Uniprot ID: P02511
• Protein Accession #: NP_001276736.1
• Nucleotide Accession #: NM_001289807.1