Catalog No: OKCD01339
Size:96 Wells
Price: $500.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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CRP High Sensitivity ELISA Kit (Human) (OKCD01339)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Serum (n=5)96-105%81-93%85-99%86-101%
EDTA Plasma (n=5)84-92%78-103%97-104%93-105%
Heparin Plasma (n=5)92-99%91-98%79-90%86-104%
ELISA Kit PrincipleAviva Systems Biology CRP ELISA Kit (Human) (OKCD01339) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells,incubated and removed. A biotinylated detector antibody specific for CRP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CRP captured in well.
ELISA Kit Range62.50-4,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-9288
EDTA Plasma (n=5)84-10188
Heparin Plasma (n=5)79-9683
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CRP were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CRP were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-CRP Microplate96 Wells (12 x 8 Well Strips)
CRP Lyophilized Standard2
100X Biotinylated CRP Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids.
Sensitivity23.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP). No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.

Association between the Inflammatory Potential of Diet and Stress among Female College Students. Nutrients. 12, NULL (2020). 32785011

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCRP
Gene Full NameC-reactive protein
Alias SymbolsPTX1
NCBI Gene Id1401
Protein NameC-reactive protein
Description of TargetDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Uniprot IDP02741
Protein Accession #NP_000558.2
Nucleotide Accession #NM_000567.2
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