CRP High Sensitivity ELISA Kit (Human) (OKCD01339)

Catalog No: OKCD01339

Size:96 Wells

CRP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-105%	81-93%	85-99%	86-101%
  EDTA Plasma (n=5)	84-92%	78-103%	97-104%	93-105%
  Heparin Plasma (n=5)	92-99%	91-98%	79-90%	86-104%

• ELISA Kit Principle: Aviva Systems Biology CRP ELISA Kit (Human) (OKCD01339) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells,incubated and removed. A biotinylated detector antibody specific for CRP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CRP captured in well.
• ELISA Kit Range: 62.50-4,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-92	88
  EDTA Plasma (n=5)	84-101	88
  Heparin Plasma (n=5)	79-96	83

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CRP were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CRP were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-CRP Microplate: 96 Wells (12 x 8 Well Strips)
  • CRP Lyophilized Standard: 2
  • 100X Biotinylated CRP Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids.
• Sensitivity: 23.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP). No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.
• Publications:

  Association between the Inflammatory Potential of Diet and Stress among Female College Students. Nutrients. 12, NULL (2020). 32785011

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CRP
• Gene Full Name: C-reactive protein
• Alias Symbols: PTX1
• NCBI Gene Id: 1401
• Protein Name: C-reactive protein
• Description of Target: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
• Uniprot ID: P02741
• Protein Accession #: NP_000558.2
• Nucleotide Accession #: NM_000567.2