CRP High Sensitivity ELISA Kit (Human) (OKCD01339)

Catalog No: OKCD01339

Size:96WELLS

CRP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-105%	81-93%	85-99%	86-101%
  EDTA Plasma (n=5)	84-92%	78-103%	97-104%	93-105%
  Heparin Plasma (n=5)	92-99%	91-98%	79-90%	86-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive C Reactive Protein (CRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive C Reactive Protein (CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive C Reactive Protein (CRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-92	88
  EDTA Plasma (n=5)	84-101	88
  Heparin Plasma (n=5)	79-96	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive C Reactive Protein (CRP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CRP Microplate: 96 Wells (12 x 8 Well Strips)
  • CRP Lyophilized Standard: 2
  • 100X Biotinylated CRP Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids.
• Sensitivity: 23.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP). No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.
• Publications:

  Association between the Inflammatory Potential of Diet and Stress among Female College Students. Nutrients. 12, NULL (2020). 32785011

  More...

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CRP
• Gene Full Name: C-reactive protein
• Alias Symbols: C-reactive protein;C-reactive protein, pentraxin-related;pentraxin 1;PTX1.
• NCBI Gene Id: 1401
• Protein Name: C-reactive protein
• Description of Target: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
• Uniprot ID: P02741
• Protein Accession #: NP_000558.2
• Nucleotide Accession #: NM_000567.2