CRP ELISA Kit (Rabbit) (OKCD04454)

Catalog No: OKCD04454

Size:96WELLS

Rabbit CRP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-90%	93-101%	97-105%	78-98%
  EDTA Plasma (n=5)	93-102%	91-101%	83-97%	83-101%
  Heparin Plasma (n=5)	80-98%	81-91%	93-103%	78-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein (CRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C Reactive Protein (CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C Reactive Protein (CRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	95-103	99
  EDTA Plasma (n=5)	92-101	95
  Heparin Plasma (n=5)	81-104	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • C3 Microplate: 96 Wells (12 x 8 Well strips)
  • C3 Lyophilized Standard: 2
  • 100X C3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 33 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CRP
• Gene Full Name: C-reactive protein
• Alias Symbols: C-reactive protein;C-reactive protein, pentraxin-related;PTX1.
• NCBI Gene Id: 100009091
• Protein Name: C-reactive protein
• Description of Target: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity).
• Uniprot ID: P02742
• Protein Accession #: NP_001075734.1
• Nucleotide Accession #: NM_001082265.1