Catalog No: OKAG00214 (Formerly GWB-SKR214)
Price: $500.00
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human CRP ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human CRP concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human CRP while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range16-1000 pg/ml
ELISA Kit Component
Capture Antibody Coated Microplate96 Wells (12 x 8)
Protein StandardLyophilized (2.5 ng)
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity16 pg/mL
SpecificityThe Human CRP ELISA is capable of recognizing both recombinant and naturally produced Human CRP proteins. The antigens listed below were tested at 60 ng/ml and exhibited less than 2% cross reactivity.
• Human: Pentraxin 2, Pentraxin 3
• Murine: CRP
• Rat: CRP
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolCRP
Gene Full NameC-reactive protein
Alias SymbolsC-reactive protein;C-reactive protein, pentraxin-related;pentraxin 1;PTX1.
NCBI Gene Id1401
Protein NameP02741
Description of TargetC-reactive protein
Uniprot IDhttps://www.uniprot.org/uniprot/P02741
Protein Accession #NP_000558.2
Nucleotide Accession #NM_000567.2
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