CRP ELISA Kit (Bovine) (OKCD06859)

Catalog No: OKCD06859

Size:96 Wells

CRP ELISA Kit (Bovine) (OKCD06859) Standard Curve

Product Info

• Predicted Species Reactivity: Bovine
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CRP ELISA Kit (Bovine) (OKCD06859) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRP captured in the well.
• ELISA Kit Range: 6.25-400ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CRP. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CRP using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • CRP Microplate: 96 Wells (12 x 8 Well strips)
  • CRP Lyophilized Standard: 2
  • 100X CRP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 2.43ng/mL
• Publications:

  Interleukin 8 and Pentaxin (C-Reactive Protein) as Potential New Biomarkers of Bovine Tuberculosis. J Clin Microbiol. 57, (2019). 31340991

Target Info

• Gene Symbol: CRP
• Gene Full Name: C-reactive protein
• Alias Symbols: C-RP; PTX1; Pentraxin-Related; Pentraxin 1
• NCBI Gene Id: 527553
• Protein Name: C-reactive protein
• Description of Target: The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli.
• Uniprot ID: C4T8B4
• Protein Accession #: NP_001137569.1
• Nucleotide Accession #: NM_001144097.1