CRP Chemi-Luminescent ELISA Kit (Rat) (OKCD03396)

Catalog No: OKCD03396

Size:96WELLS

Rat CRP ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-101%	92-102%	84-91%	87-101%
  EDTA Plasma (n=5)	98-105%	78-96%	97-105%	88-97%
  Heparin Plasma (n=5)	87-95%	78-88%	82-103%	85-102%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein (CRP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the C Reactive Protein (CRP) level in the sample or standard.
• ELISA Kit Range: 0.14-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-93	83
  EDTA Plasma (n=5)	80-97	91
  Heparin Plasma (n=5)	95-104	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Crp Microplate: 96 Wells (12 x 8 Well strips)
  • Crp Lyophilized Standard: 2
  • 100X Crp Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.14 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Crp
• Gene Full Name: C-reactive protein
• Alias Symbols: Aa1249;Ab1-341;Ab2-196;Ac1-114;Ac1262;Ac2-069;Ba2-693;C-reactive protein;C-reactive protein, member of the pentraxin family;C-reactive protein, pentraxin-related;C-reactive protein, petaxin related.
• NCBI Gene Id: 25419
• Protein Name: C-reactive protein
• Description of Target: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity).
• Uniprot ID: P48199
• Protein Accession #: NP_058792
• Nucleotide Accession #: NM_017096