CRLF1 ELISA Kit (Human) (OKCD08768)

Catalog No: OKCD08768

Size:96WELLS

CRLF1 ELISA Kit (Human) (OKCD08768) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokine Receptor Like Factor 1 (CRLF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokine Receptor Like Factor 1 (CRLF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokine Receptor Like Factor 1 (CRLF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokine Receptor Like Factor 1 (CRLF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokine Receptor Like Factor 1 (CRLF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokine Receptor Like Factor 1 (CRLF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CRLF1 Microplate: 96 Wells (12 x 8 Well strips)
  • CRLF1 Lyophilized Standard: 2
  • 100X CRLF1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.053ng/mL

Target Info

• Gene Symbol: CRLF1
• Gene Full Name: cytokine receptor like factor 1
• Alias Symbols: CISS;CISS1;class I cytokine receptor;CLF;CLF-1;cytokine receptor-like factor 1;cytokine type 1 receptor CRLP-1;cytokine-like factor 1;NR6;zcytor5.
• NCBI Gene Id: 9244
• Protein Name: Cytokine receptor-like factor 1
• Description of Target: Cytokine receptor subunit, possibly playing a regulatory role in the immune system and during fetal development. May be involved in nervous system development.
• Uniprot ID: O75462
• Protein Accession #: NP_004741.1
• Nucleotide Accession #: NM_004750.4