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Catalog No: OKCD00515 (Formerly GWB-KBBWS6)
Size:96WELLS
Price: $675.00
SKU
OKCD00515
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Datasheets/ManualsPrintable datasheet for CRKL ELISA Kit (Human) (OKCD00515)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crk Like Protein (CRKL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crk Like Protein (CRKL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crk Like Protein (CRKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Crk Like Protein (CRKL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Crk Like Protein (CRKL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Crk Like Protein (CRKL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CRKL Microplate96 Wells (12 x 8 Well Strips)
CRKL Lyophilized Standard2 x 10 ng
100X Biotinylated CRKL Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Crk Like Protein (CRKL). No significant cross-reactivity or interference between Crk Like Protein (CRKL) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCRKL
Gene Full NameCRK like proto-oncogene, adaptor protein
Alias Symbolscrk-like protein;v-crk avian sarcoma virus CT10 oncogene homolog-like.
NCBI Gene Id1399
Protein NameCrk-like protein
Description of TargetMay mediate the transduction of intracellular signals.
Uniprot IDP46109
Protein Accession #NP_005198
Nucleotide Accession #NM_005207.3
Protein Size (# AA)303
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