Catalog No: OKCD08775
Size:96 Wells
Price: $800.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CRISP3 ELISA Kit (Human) (OKCD08775) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRISP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRISP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRISP3 captured in the well.
ELISA Kit Range0.94-60ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CRISP3. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CRISP3 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
CRISP3 Microplate96 Wells (12 x 8 Well strips)
CRISP3 Lyophilized Standard2
100X CRISP3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, saliva, sweat and other biological fluids.
Sensitivity< 0.43ng/mL
Gene SymbolCRISP3
Gene Full Namecysteine rich secretory protein 3
Alias SymbolsAeg2, CRS3, SGP28, CRISP-3, dJ442L6.3
NCBI Gene Id10321
Protein NameCysteine-rich secretory protein 3
Description of TargetThe function of this protein remains unknown.
Uniprot IDP54108
Protein Accession #NP_001177915.1
Nucleotide Accession #NM_001190986.2
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