Catalog No: OKBB00579
Size:96 Wells
Price: $583.00
SKU
OKBB00579
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human CRISP3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NS0, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CRISP3 amount of sample captured in plate.
ELISA Kit Range62.5 pg/ml - 4,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)184172203865
SD0.631.72.741.142.43.8
CV%3.54.13.85.76.35.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human CRISP3 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human CRISP3 Standard2 x 100 ng/tube
100X Biotinylated Anti-Human CRISP3 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenates
Sensitivity<50 pg/ml
SpecificityNatural and recombinant human CRISP3
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: N21-Y245
DescriptionFor quantitative detection of human CRISP3 in cell culture supernatants, serum, plasma(heparin, EDTA), saliva and urine.
Gene SymbolCRISP3
Gene Full Namecysteine-rich secretory protein 3
Alias SymbolsAeg 2, Aeg2, CRIS3_HUMAN, CRISP 3, CRISP-3, Crisp3, CRS 3, CRS3, Cysteine rich secretory protein 3, Cysteine-rich secretory protein 3, dJ442L6.3, MGC126588, OTTHUMP00000016590, OTTHUMP00000039911, SGP 28, SGP28, SGP28 protein, Specific granule protein (28 kDa), Specific granule protein of 28 kDa
NCBI Gene Id10321
Protein NameCysteine-rich secretory protein 3
Description of TargetCysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.
Uniprot IDP54108
Protein Accession #NP_001177915.1
Nucleotide Accession #NM_001190986.1
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