Catalog No: OKBB00579
Size:96 Wells
Price: $583.00
SKU
OKBB00579
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||||
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Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva's human CRISP3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NS0, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CRISP3 amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Range | 62.5 pg/ml - 4,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
Sample Type | Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenates | ||||||||||||||||||||||||||||||||||||||||||
Sensitivity | <50 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
Specificity | Natural and recombinant human CRISP3 | ||||||||||||||||||||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
Immunogen | Expression system for standard: NS0; Immunogen sequence: N21-Y245 | ||||||||||||||||||||||||||||||||||||||||||
Description | For quantitative detection of human CRISP3 in cell culture supernatants, serum, plasma(heparin, EDTA), saliva and urine. |
Gene Symbol | CRISP3 |
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Gene Full Name | cysteine-rich secretory protein 3 |
Alias Symbols | Aeg 2, Aeg2, CRIS3_HUMAN, CRISP 3, CRISP-3, Crisp3, CRS 3, CRS3, Cysteine rich secretory protein 3, Cysteine-rich secretory protein 3, dJ442L6.3, MGC126588, OTTHUMP00000016590, OTTHUMP00000039911, SGP 28, SGP28, SGP28 protein, Specific granule protein (28 kDa), Specific granule protein of 28 kDa |
NCBI Gene Id | 10321 |
Protein Name | Cysteine-rich secretory protein 3 |
Description of Target | Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity. |
Uniprot ID | P54108 |
Protein Accession # | NP_001177915.1 |
Nucleotide Accession # | NM_001190986.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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