CRIM1 ELISA Kit (Human) (OKCD01996)

Catalog No: OKCD01996 (Formerly GWB-KBBSB6)

Size:96WELLS

Human CRIM1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	94-105%	89-103%	90-98%	97-104%
  EDTA Plasma (n=5)	98-105%	82-101%	89-98%	82-94%
  Heparin Plasma (n=5)	80-98%	89-103%	78-89%	95-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	90-99	95
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma (n=5)	95-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CRIM1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CRIM1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CRIM1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1). No significant cross-reactivity or interference between Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CRIM1
• Gene Full Name: cysteine rich transmembrane BMP regulator 1
• Alias Symbols: CRIM-1;cysteine rich transmembrane BMP regulator 1 (chordin-like);cysteine-rich motor neuron 1 protein;cysteine-rich repeat-containing protein S52;S52.
• NCBI Gene Id: 51232
• Protein Name: Cysteine-rich motor neuron 1 protein
• Description of Target: May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface.
• Uniprot ID: Q9NZV1
• Protein Accession #: NP_057525.1
• Nucleotide Accession #: NM_016441.2
• Protein Size (# AA): 1036