CRHR2 ELISA Kit (Human) (OKCD08495)

Catalog No: OKCD08495

Size:96WELLS

CRHR2 ELISA Kit (Human) (OKCD08495) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticotropin Releasing Hormone Receptor 2 (CRHR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone Receptor 2 (CRHR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticotropin Releasing Hormone Receptor 2 (CRHR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Corticotropin Releasing Hormone Receptor 2 (CRHR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 2 (CRHR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 2 (CRHR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CRHR2 Microplate: 96 Wells (12 x 8 Well strips)
  • CRHR2 Lyophilized Standard: 2
  • 100X CRHR2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.115ng/mL

Target Info

• Gene Symbol: CRHR2
• Gene Full Name: corticotropin releasing hormone receptor 2
• Alias Symbols: CRF2, CRFR2, CRF-RB, HM-CRF
• NCBI Gene Id: 1395
• Protein Name: Corticotropin-releasing factor receptor 2
• Description of Target: CRHR2 is a receptor for corticotropin releasing factor.It Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
• Uniprot ID: Q13324
• Protein Accession #: NP_001189404.1
• Nucleotide Accession #: NM_001202475.1