CRHR1 ELISA Kit (Rat) (OKCD08319)

Catalog No: OKCD08319

Size:96WELLS

CRHR1 ELISA Kit (Rat) (OKCD08319) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Corticotropin Releasing Hormone Receptor 1 (CRHR1) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Corticotropin Releasing Hormone Receptor 1 (CRHR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 1 (CRHR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 1 (CRHR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CRHR1 Microplate: 96 Wells (12 x 8 Well strips)
  • CRHR1 Lyophilized Standard: 2
  • 100X CRHR1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.054ng/mL

Target Info

• Gene Symbol: Crhr1
• Gene Full Name: corticotropin releasing hormone receptor 1
• Alias Symbols: corticotrophin releasing hormone receptor 1;corticotropin releasing hormone 1;corticotropin-releasing factor receptor 1;Corticotropin-releasing hormone receptor 1;CRF1;CRF-R;CRFR1;CRFR-1;CRF-R1;CRF-R-1;CRH-R 1;CRH-R1;CRH-R-1.
• NCBI Gene Id: 58959
• Protein Name: Corticotropin-releasing factor receptor 1
• Description of Target: G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.
• Uniprot ID: P35353
• Protein Accession #: NP_112261.1
• Nucleotide Accession #: NM_030999.4