Catalog No: OKEH00624
Size:96 Wells
Price: $600.00
SKU
OKEH00624
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CRH ELISA Kit (Mouse) : 96 Wells (OKEH00624)

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00624
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Crh ELISA Kit (Mouse) (OKEH00624) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog Crh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Crh and incubated. The Crh found in the sample or standards competes with the biotinylated Crh for limited binding sites on the immobilized anti-dog Crh antibody. Excess unbound biotinylated Crh and sample or standard Crh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Crh captured in the well and inversely proportional to the amount of Crh which was contained in the sample or standard.
ELISA Kit Range15.6 - 1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CRH Microplate96 Wells (12 x 8 Well strips)
CRH Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Crh-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Mouse Corticoliberin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCRH
Gene Full Namecorticotropin releasing hormone
Alias SymbolsCR, CRF, Gm1347
NCBI Gene Id12918
Protein NameCorticoliberin
Description of TargetThis gene encodes a member of the corticotropin-releasing factor family and preproprotein that is proteolytically processed to generate a mature protein product. This protein product is a neuropeptide hormone that binds to the corticotropin releasing hormone receptors (CRHR1 and CRHR2) to stimulate the release of adrenocorticotropic hormone from the pituitary gland in response to stress. The encoded protein may also regulate angiogenesis and inflammation. Homozygous knockout mice for this gene exhibit reduced corticosterone levels while the offspring of these mice die perinatally. [provided by RefSeq, Aug 2015]
Swissprot IdQ8CIT0
Protein Accession #NP_991338.1
Nucleotide Accession #NM_205769.3
Protein Size (# AA)187
Molecular Weight20 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CRH.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CRH.
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