CRH ELISA Kit (Mouse) : 96 Wells (OKEH00624)

Catalog No: OKEH00624

Size:96T

Mouse CRH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: ELISA-Comp
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Crh ELISA Kit (Mouse) (OKEH00624) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog Crh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Crh and incubated. The Crh found in the sample or standards competes with the biotinylated Crh for limited binding sites on the immobilized anti-dog Crh antibody. Excess unbound biotinylated Crh and sample or standard Crh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Crh captured in the well and inversely proportional to the amount of Crh which was contained in the sample or standard.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 8.4% (n = 20)
• ELISA Kit Component:
  • Anti-CRH Microplate: 96 Wells (12 x 8 Well strips)
  • CRH Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Crh-Biotin Complex: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 7.8 pg/mL
• Specificity: Natural and recombinant Mouse Corticoliberin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Crh
• Gene Full Name: corticotropin releasing hormone
• Alias Symbols: corticoliberin;corticotropin releasing factor;Corticotropin-releasing factor;Corticotropin-releasing hormone;CR;CRF;Gm1347.
• NCBI Gene Id: 12918
• Protein Name: Q8CIT0
• Description of Target: Corticoliberin
• Uniprot ID: https://www.uniprot.org/uniprot/Q8CIT0
• Protein Accession #: NP_991338.1
• Nucleotide Accession #: NM_205769.3
• Protein Size (# AA): 187
• Molecular Weight: 20 kDa