Size:96 Wells
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CRH ELISA Kit (Mouse) : 96 Wells (OKEH00624)

100% of 100
Catalog#: OKEH00624
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology Crh ELISA Kit (Mouse) (OKEH00624) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog Crh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Crh and incubated. The Crh found in the sample or standards competes with the biotinylated Crh for limited binding sites on the immobilized anti-dog Crh antibody. Excess unbound biotinylated Crh and sample or standard Crh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Crh captured in the well and inversely proportional to the amount of Crh which was contained in the sample or standard.
Kit Range 15.6 - 1000pg/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Kit Reproducibility Mean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
Kit Component
Anti-CRH Microplate96 Wells (12 x 8 Well strips)
CRH Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Crh-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity 7.8 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA Datasheet Printable COA datasheet for OKEH00624
Specificity Natural and recombinant Mouse Corticoliberin
Assay Info Assay Methodology: Quantitative Competitive ELISA
Gene Symbol CRH
Official Gene Full Name corticotropin releasing hormone
Alias Symbols Corticoliberin, corticotropin releasing factor, corticotropin-releasing factor, Corticotropin-releasing factor, Corticotropin-releasing hormone, CRF, Gm1347, LOC383938, MGC151298
NCBI Gene Id 12918
Protein Name Corticoliberin
Description of Target This gene encodes a member of the corticotropin-releasing factor family and preproprotein that is proteolytically processed to generate a mature protein product. This protein product is a neuropeptide hormone that binds to the corticotropin releasing hormone receptors (CRHR1 and CRHR2) to stimulate the release of adrenocorticotropic hormone from the pituitary gland in response to stress. The encoded protein may also regulate angiogenesis and inflammation. Homozygous knockout mice for this gene exhibit reduced corticosterone levels while the offspring of these mice die perinatally. [provided by RefSeq, Aug 2015]
Swissprot Id Q8CIT0
Protein Accession # NP_991338.1
Nucleotide Accession # NM_205769.3
Protein Size (# AA) 187
Molecular Weight 20 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CRH.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CRH.
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