
Catalog No: OKEH00623
Size:96T
Price: $650.00
SKU
OKEH00623
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
CRH ELISA Kit (Human) : 96 Wells (OKEH00623)
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CRH ELISA Kit (Human) (OKEH00623) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRH captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.3% (n = 20) Mean Inter-assay CV%: < 8.9% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 3.9 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Corticoliberin | ||||||||||||||||||||||
Publications | LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion. Front Mol Neurosci. 11, 352 (2018). 30337854 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
Description |
Gene Symbol | CRH |
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Gene Full Name | corticotropin releasing hormone |
Alias Symbols | corticoliberin;corticotropin-releasing factor;Corticotropin-releasing hormone;CRF;CRH1. |
NCBI Gene Id | 1392 |
Protein Name | P06850 |
Description of Target | Corticoliberin |
Uniprot ID | https://www.uniprot.org/uniprot/P06850 |
Protein Accession # | NP_000747.1 |
Nucleotide Accession # | NM_000756.3 |
Protein Size (# AA) | 196 |
Molecular Weight | 21 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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