CRH ELISA Kit (Human) : 96 Wells (OKEH00623)

Catalog No: OKEH00623

Size:96 Wells

Human CRH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CRH ELISA Kit (Human) (OKEH00623) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRH captured in well.
  
• ELISA Kit Range: 15.6 - 1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.3% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-CRH Microplate: 96 Wells (12 x 8 Well strips)
  • CRH Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CRH Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 3.9 pg/mL
• Specificity: Natural and recombinant Human Corticoliberin
• Publications:

  LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion. Front Mol Neurosci. 11, 352 (2018). 30337854

• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CRH
• Gene Full Name: corticotropin releasing hormone
• Alias Symbols: CRF, CRH1
• NCBI Gene Id: 1392
• Protein Name: Corticoliberin
• Description of Target: This gene encodes a member of the corticotropin-releasing factor family. The encoded preproprotein is proteolytically processed to generate the mature neuropeptide hormone. In response to stress, this hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus, binds to corticotropin releasing hormone receptors and stimulates the release of adrenocorticotropic hormone from the pituitary gland. Marked reduction in this protein has been observed in association with Alzheimer's disease. Autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Nov 2015]
• Uniprot ID: P06850
• Protein Accession #: NP_000747.1
• Nucleotide Accession #: NM_000756.3
• Protein Size (# AA): 196
• Molecular Weight: 21 kDa