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Catalog No: OCOA04284
Size:20UG
Price: $179.00
SKU
OCOA04284
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CREM Protein Lysate (OCOA04284)

Datasheets/ManualsPrintable datasheet for CREM Protein Lysate (OCOA04284)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCREM HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCREM
Gene Full NamecAMP responsive element modulator
Alias SymbolscAMP response element modulator;cAMP-responsive element modulator;CREM 2alpha-b protein;CREM 2beta-a protein;CREM-2;hCREM-2;ICER;Inducible cAMP early repressor;inducible cAMP early repressor ICER.
NCBI Gene Id1390
Protein NameNM_183011, NP_898829, Q03060
Description of TargetcAMP-responsive element modulator, cAMP-responsive element modulator isoform 21 [Homo sapiens], Homo sapiens cAMP responsive element modulator (CREM), transcript variant 21, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q03060
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_898829
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_183011
Molecular Weight32.3 kDa
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