CRELD1 ELISA Kit (Human) (OKCA02092)

Catalog No: OKCA02092

Size:96 Wells

Human CRELD1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	92	87-96
  Serum (n=4)	1:2	95	90-100
  Serum (n=4)	1:4	90	85-94
  Serum (n=4)	1:8	106	100-113

• ELISA Kit Principle: Aviva Systems Biology CRELD1 ELISA Kit (Human) (OKCA02092) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRELD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRELD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CRELD1 captured in well.
• ELISA Kit Range: 23.5 pg/mL-1,500 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	89	85-93
  EDTA plasma (n=4)	98	93-104

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • CRELD1 Microplate: 96 Wells (12 x 8 Well strips)
  • CRELD1 Lyophilized Standard: 2 x 1.5 ng
  • 100X Biotinylated CRELD1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • (???)X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 5.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CRELD1
• Alias Symbols: AVSD2, CIRRIN
• NCBI Gene Id: 78987
• Protein Name: Cysteine-rich with EGF-like domain protein 1
• Uniprot ID: Q96HD1
• Protein Accession #: NP_001026887
• Nucleotide Accession #: NM_001031717