CREG1 ELISA Kit (Mouse) (OKDD00755)

Catalog No: OKDD00755 (Formerly GWB-KBBCN5)

Size:96 Wells

Mouse CREG1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology CREG1 ELISA Kit (Mouse) (OKDD00755) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CREG1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CREG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CREG1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-CREG1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CREG1 Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X CREG1 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates
• Sensitivity: <0.053ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of CREG1. No significant cross-reactivity or interference between CREG1 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Creg1
• Gene Full Name: cellular repressor of E1A-stimulated genes 1
• Alias Symbols: AA755314;Cellular repressor of E1A-stimulated genes 1;Cre;Creg;protein CREG1.
• NCBI Gene Id: 433375
• Protein Name: Protein CREG1
• Description of Target: May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R (By similarity).
• Uniprot ID: O88668
• Protein Accession #: NP_035934.1
• Nucleotide Accession #: NM_011804.2
• Protein Size (# AA): 220
• Molecular Weight: 24 kDa