CREG1 ELISA Kit (Human) (OKCD01089)

Catalog No: OKCD01089 (Formerly GWB-KBBPB5)

Size:96WELLS

Human CREG1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cellular Repressor Of E1A Stimulated Genes 1 (CREG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cellular Repressor Of E1A Stimulated Genes 1 (CREG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cellular Repressor Of E1A Stimulated Genes 1 (CREG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CREG1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CREG1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CREG1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cellular Repressor Of E1A Stimulated Genes 1 (CREG1). No significant cross-reactivity or interference between Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CREG1
• Gene Full Name: cellular repressor of E1A stimulated genes 1
• Alias Symbols: Cellular repressor of E1A-stimulated genes 1;CREG;protein CREG1.
• NCBI Gene Id: 8804
• Protein Name: Protein CREG1
• Description of Target: May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.
• Uniprot ID: O75629
• Protein Accession #: NP_003842
• Nucleotide Accession #: NM_003851.2
• Protein Size (# AA): 220