Catalog No: OKCD00928 (Formerly GWB-KBBCQ5)
Size:96WELLS
Price: $675.00
SKU
OKCD00928
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Datasheets/ManualsPrintable datasheet for CREBBP ELISA Kit (Human) (OKCD00928)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CREB Binding Protein (CREBBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CREB Binding Protein (CREBBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CREB Binding Protein (CREBBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CREB Binding Protein (CREBBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CREB Binding Protein (CREBBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CREB Binding Protein (CREBBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CREBBP Microplate96 Wells (12 x 8 Well Strips)
CREBBP Lyophilized Standard2 x 20 ng
100X Biotinylated CREBBP Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.132 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of CREB Binding Protein (CREBBP). No significant cross-reactivity or interference between CREB Binding Protein (CREBBP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCREBBP
Gene Full NameCREB binding protein
Alias SymbolsCBP;CREB-binding protein;histone lysine acetyltransferase CREBBP;KAT3A;MKHK1;protein-lysine acetyltransferase CREBBP;RSTS;RSTS1.
NCBI Gene Id1387
Protein NameCREB-binding protein
Description of TargetAcetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902).
Uniprot IDQ92793
Protein Accession #NP_001073315
Nucleotide Accession #NM_001079846.1
Protein Size (# AA)2442
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