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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for anti-CREBBP (OASG01132) antibody |
|---|
| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB, IHC, IF |
| Additional Information | Cellular Localization: Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. |
| Reconstitution and Storage | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Immunogen | Synthesized peptide derived between 1480-1550 aa from human CBP around the non-acetylation site of K1535. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Target Post-Translational Modification | Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response . Phosphorylated by CHUK/IKKA at Ser-1382 and Ser-1386; these phosphorylations promote cell growth by switching the binding preference of CREBBP from TP53 to NF-kappa-B. Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX. Autoacetylation is required for binding to protein substrates, such as acetylated histones and acetylated TP53/p53. |
| Specificity | CBP Polyclonal Antibody detects endogenous levels of CBP protein. |
| Application Info | WB: 1:500-1:2000 IHC: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:20000 Optimal dilutions should be determined by the end user. |
| Gene Symbol | CREBBP |
|---|---|
| Gene Full Name | CREB binding protein |
| Alias Symbols | CBP, RSTS, KAT3A, MKHK1, RSTS1 |
| NCBI Gene Id | 1387 |
| Description of Target | Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) . |
| Uniprot ID | Q92793, P45481, Q6JHU9 |
| Molecular Weight | 265 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CREBBP Antibody (OASG01132)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "CREBBP Antibody (OASG01132)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "CREBBP Antibody (OASG01132)" provided in?
This item is provided in "Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CREBBP Antibody (OASG01132)"?
This target may also be called "CBP, RSTS, KAT3A, MKHK1, RSTS1" in publications.
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What is the shipping cost for "CREBBP Antibody (OASG01132)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CREBBP Antibody (OASG01132)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CREBBP Antibody (OASG01132)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "265 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CREBBP Antibody (OASG01132)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CREBBP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CREBBP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CREBBP"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CREBBP"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CREBBP"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CREBBP"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
