Catalog No: OKEH04003
Size:96T
Price: $375.00
SKU
OKEH04003
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04003
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CREB1 ELISA Kit (Human) (OKEH04003) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CREB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CREB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CREB1 captured in the well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CREB1 Microplate96 Wells (12 x 8 Well Strips)
CREB1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
CREB1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.98 ng/mL
SpecificityNatural and recombinant Human Cyclic AMP-responsive element-binding protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCREB1
Gene Full NamecAMP responsive element binding protein 1
Alias Symbolsactive transcription factor CREB;cAMP-response element-binding protein-1;CREB;CREB-1;cyclic adenosine 3',5'-monophosphate response element binding protein;cyclic adenosine 3',5'-monophosphate response element-binding protein CREB;cyclic AMP-responsive element-binding protein 1;transactivator protein.
NCBI Gene Id1385
Protein NameP16220
Description of TargetCyclic AMP-responsive element-binding protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/P16220
Protein Accession #NP_004370.1
Nucleotide Accession #NM_004379.4
Protein Size (# AA)341
Molecular Weight37 kDa
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