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Creb1 Antibody (OASA07185)

Catalog#: OASA07185
This product is Genway GWB-3AC8CA
More Information
Predicted Species ReactivityRat
Product FormatPurified IgG - liquid
IsotypePolyclonal IgG
::Preservative Stabilisers: 0.09% - Sodium Azide
0.01% - Bovine Serum Albumin
50% - Glycerol
Antiserum Preparation: Antisera to phosphorylated rat CREB were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
::Buffer Solutions: 10mM HEPES pH7.5
Reconstitution and StorageStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Replacement ItemThis antibody may replace item sc-186 from Santa Cruz Biotechnology.
ImmunogenThe immunogen for anti-Creb1 antibody: synthetic phosphopeptide corresponding to an amino acid sequence within CREB which includes phosphorylated Ser133.
Predicted Homology Based on Immunogen SequenceRat
Datasheets/ManualsPrintable datasheet for anti-Creb1 antibody - OASA07185
Application InfoWestern Blot: This product detects a band of approximately 45kDa in rat hippocampus lysates that had been incubated in the presence of forskolin.
* For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Target Reference1. Mayr, B.M. et al. (2001) Distinct effects of cAMP and mitogenic signals on CREB-binding protein recruitment impart specificity to target gene activation via CREB. Proc. Natl. Acad. Sci. USA 98: 10936 - 10941.
Gene SymbolCreb1
Alias SymbolsCreb, Creb1
NCBI Gene Id81646
Protein NameCyclic AMP-responsive element-binding protein 1
Description of TargetRABBIT ANTI CREB (pSer133)
Swissprot IdP15337
Protein Accession #NP_112279.1
Protein Size (# AA)341
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Creb1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Creb1.
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