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CPT2 ELISA Kit (Human) (OKCD00869)

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Application:
ELISA
Gene Symbol:
CPT2
Official Gene Full Name:
carnitine palmitoyltransferase 2
NCBI Gene Id:
1376
Alias Symbols:
Carnitine O-palmitoyltransferase 2, mitochondrial, Carnitine palmitoyltransferase II, CPT1, CPTASE, CPT II
Protein Name:
Carnitine O-palmitoyltransferase 2, mitochondrial
Swissprot Id:
P23786
Protein Accession #:
NP_000089
Nucleotide Accession #:
NM_000098.2
Predicted Species Reactivity:
Human
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
< 0.114 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level CPT2 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CPT2 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology CPT2 ELISA Kit (Human) (OKCD00869) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPT2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CPT2captured in well.
Kit Component:
ComponentAmount
Anti-CPT2 Microplate96 Wells (12 x 8 Well Strips)
CPT2 Lyophilized Standard2 x 20 ng
100X Biotinylated CPT2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CPT2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CPT2.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2). No significant cross-reactivity or interference between Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
658

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