Catalog No: OKCA01639
Size:96 Wells
Price: $754.00
SKU
OKCA01639
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat9788-101
1:210597-109
1:410088-104
1:89485-98
ELISA Kit PrincipleAviva Systems Biology Cps1 ELISA Kit (Mouse) (OKCA01639) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cps1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cps1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cps1 captured in the well.
ELISA Kit Range6.25 - 400 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)10492-107
EDTA Plasma (n=4)10091-104
ELISA Kit Component
ComponentAmount
Cps1 Microplate96 Wells (12 x 8 Well strips)
Cps1 Lyophilized Standard2 x 400 pg
100X Biotinylated Cps1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity1.56 pg/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolCPS1
Gene Full Namecarbamoyl-phosphate synthetase 1
Alias SymbolsC, CPS, D1Ucl, D1Ucla3, 4732433M03Rik
NCBI Gene Id227231
Protein NameCarbamoyl-phosphate synthase [ammonia], mitochondrial
Description of TargetInvolved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
Uniprot IDQ8C196
Protein Accession #NP_001074278.1
Nucleotide Accession #NM_001080809.2
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