Catalog No: OKCD01141 (Formerly GWB-KBBQS2)
Size:96WELLS
Price: $700.00
SKU
OKCD01141
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Datasheets/ManualsPrintable datasheet for CPN1 ELISA Kit (Mouse) (OKCD01141)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)91-98%79-105%95-104%96-105%
EDTA plasma(n=5)80-101%89-103%80-90%93-103%
heparin plasma(n=5)84-99%98-105%98-105%97-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase N1 (CPN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase N1 (CPN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase N1 (CPN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxypeptidase N1 (CPN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range62.5-4,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-10198
EDTA plasma(n=5)78-9287
heparin plasma(n=5)86-9891
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase N1 (CPN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase N1 (CPN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cpn1 Microplate96 Wells (12 x 8 Well Strips)
Cpn1 Lyophilized Standard2 x 4 ng
100X Biotinylated Cpn1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 24.2 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase N1 (CPN1). No significant cross-reactivity or interference between Carboxypeptidase N1 (CPN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCpn1
Gene Full Namecarboxypeptidase N, polypeptide 1
Alias Symbols0610011F20Rik;anaphylatoxin inactivator;C;carboxypeptidase N catalytic chain;Carboxypeptidase N polypeptide 1;carboxypeptidase N small subunit;carboxypeptidase N, polypeptide 1, 50kD;CPN;kininase I.
NCBI Gene Id93721
Protein NameCarboxypeptidase N catalytic chain
Description of TargetProtects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
Uniprot IDQ9JJN5
Protein Accession #NP_001299
Nucleotide Accession #NM_001308.2
Protein Size (# AA)457
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