CPM ELISA Kit (Mouse) (OKCA02351)

Catalog No: OKCA02351

Size:96 Wells

Mouse CPM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  1:100	87	83-91
  1:200	104	99-108
  1:400	92	87-98
  1:800	93	86-97

• ELISA Kit Principle: Aviva Systems Biology Cpm ELISA Kit (Mouse) (OKCA02351) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cpm has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cpm is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cpm captured in the well.
• ELISA Kit Range: 12.5 - 800 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	88	83-92
  EDTA Plasma (n=4)	93	87-97

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Cpm Microplate: 96 Wells (12 x 8 Well strips)
  • Cpm Lyophilized Standard: 2
  • 100X Biotinylated Cpm Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 2 x 20 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernates And Tissue Homogenates
• Sensitivity: 3.1 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CPM
• Alias Symbols: AA589379, 1110060I01Rik, 5730456K23Rik, E030045M14Rik
• NCBI Gene Id: 70574
• Protein Name: Carboxypeptidase M
• Description of Target: Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins.
• Uniprot ID: Q80V42
• Protein Accession #: NP_081744
• Nucleotide Accession #: NM_027468