Catalog No: OKCD02916
Size:96WELLS
Price: $625.00
SKU
OKCD02916
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Datasheets/ManualsPrintable datasheet for CPB2 ELISA Kit (Mouse) (OKCD02916)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
sodium citrate plasma(n=5)90-98%87-94%82-96%90-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase B2 (CPB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase B2 (CPB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase B2 (CPB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxypeptidase B2 (CPB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
sodium citrate plasma(n=5)96-10399
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase B2 (CPB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase B2 (CPB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CPB2 Microplate96 Wells (12 x 8 Well strips)
CPB2 Lyophilized Standard2
100X Biotinylated CPB2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma
Sensitivity0.128 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thrombin Activatable Fibrinolysis Inhibitor (TAFI). No significant cross-reactivity or interference between Thrombin Activatable Fibrinolysis Inhibitor (TAFI) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCpb2
Gene Full Namecarboxypeptidase B2 (plasma)
Alias Symbols1110032P04Rik;4930405E17Rik;AI255929;C;carboxypeptidase B2;carboxypeptidase R;carboxypeptidase U;CP;CPR;Cpu;TA;TAFI;thrombin-activable fibrinolysis inhibitor;thrombin-activatable fibrinolysis inhibitor.
NCBI Gene Id56373
Protein NameCarboxypeptidase B2
Description of TargetCleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.
Uniprot IDQ9JHH6
Protein Accession #NP_062749.2
Nucleotide Accession #NM_019775.3
Protein Size (# AA)422
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