CPB2 ELISA Kit (Human) (OKCD06584)

Catalog No: OKCD06584

Size:96WELLS

CPB2 ELISA Kit (Human) (OKCD06584) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase B2 (CPB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase B2 (CPB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase B2 (CPB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxypeptidase B2 (CPB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 2.5-160ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase B2 (CPB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase B2 (CPB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CPB2 Microplate: 96 Wells (12 x 8 Well strips)
  • CPB2 Lyophilized Standard: 2
  • 100X CPB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 0.133ng/mL

Target Info

• Gene Symbol: CPB2
• Gene Full Name: carboxypeptidase B2
• Alias Symbols: carboxypeptidase B2;carboxypeptidase B2 (plasma);carboxypeptidase B2 (plasma, carboxypeptidase U);carboxypeptidase B-like protein;carboxypeptidase R;Carboxypeptidase U;CPU;PCPB;Plasma carboxypeptidase B;TAFI;thrombin-activable fibrinolysis inhibitor;thrombin-activatable fibrinolysis inhibitor.
• NCBI Gene Id: 1361
• Protein Name: Carboxypeptidase B2
• Description of Target: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.
• Uniprot ID: Q96IY4
• Protein Accession #: NP_001265470.1
• Nucleotide Accession #: NM_001278541.1