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Catalog No: OKEH02184
Size:96T
Price: $625.00
SKU
OKEH02184
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CPB1 ELISA Kit (Pig) (OKEH02184) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pig CPB1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CPB1 and incubated. The CPB1 found in the sample or standards competes with the biotinylated CPB1 for limited binding sites on the immobilized anti-Pig CPB1 antibody. Excess unbound biotinylated CPB1 and sample or standard CPB1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CPB1 captured in the well and inversely proportional to the amount of CPB1 which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CPB1 Microplate96 Wells (12 x 8 Well strips)
CPB1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X CPB1 Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.254 ng/mL
SpecificityNatural and recombinant Pig Carboxypeptidase B
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCPB1
Gene Full Namecarboxypeptidase B1
Alias Symbolscarboxypeptidase B;carboxypeptidase B1 (tissue);procarboxypeptidase B.
NCBI Gene Id397341
Protein NameP09955
Description of TargetCarboxypeptidase B
Uniprot IDhttps://www.uniprot.org/uniprot/P09955
Protein Accession #NP_999334.1
Nucleotide Accession #NM_214169.1
Protein Size (# AA)416
Molecular Weight47 kDa
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