Catalog No: OKDD00203 (Formerly GWB-KBBTG4)
Size:96 Wells
Price: $800.00
SKU
OKDD00203
Availability: Domestic: within 2 weeks delivery | International: 2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology CPA4 ELISA Kit (Human) (OKDD00203) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for CPA4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CPA4 captured in well.
ELISA Kit Range0.156-10 ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPA4 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPA4 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-CPA4 Microplate96 Wells (12 x 8 Well Strips)
CPA4 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CPA4 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates
Sensitivity< 0.051 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of CPA4. No significant cross-reactivity or interference between CPA4 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolCPA4
Gene Full Namecarboxypeptidase A4
Alias SymbolsCPA3
NCBI Gene Id51200
Protein NameCarboxypeptidase A4
Description of TargetThis gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness.
Swissprot IdQ9UI42
Protein Accession #NP_001156918.1
Nucleotide Accession #NM_001163446.1
Protein Size (# AA)421
Molecular Weight47 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CPA4.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CPA4.
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