Catalog No: OKCD08770
Size:96 Wells
Price: $800.00
SKU
OKCD08770
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CPA4 ELISA Kit (Human) (OKCD08770) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPA4 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CPA4. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CPA4 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CPA4 Microplate96 Wells (12 x 8 Well strips)
CPA4 Lyophilized Standard2
100X CPA4 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.065ng/mL
Gene SymbolCPA4
Gene Full Namecarboxypeptidase A4
Alias SymbolsCPA3
NCBI Gene Id51200
Protein NameCarboxypeptidase A4
Description of TargetThis gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness.
Swissprot IdQ9UI42
Protein Accession #NP_001156918.1
Nucleotide Accession #NM_001163446.1
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CPA4.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CPA4.
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