CPA3 ELISA Kit (Human) (OKEH04688)

Catalog No: OKEH04688

Size:96T

Human CPA3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology CPA3 ELISA Kit (Human) (OKEH04688) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CPA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPA3 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-CPA3 Microplate: 96 Wells (12 x 8 Well Strips)
  • CPA3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • CPA3 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.34 ng/mL
• Specificity: Natural and recombinant Human Mast cell carboxypeptidase A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CPA3
• Gene Full Name: carboxypeptidase A3
• Alias Symbols: Carboxypeptidase A3;carboxypeptidase A3 (mast cell);mast cell carboxypeptidase A;MC-CPA;tissue carboxypeptidase A.
• NCBI Gene Id: 1359
• Protein Name: P15088
• Description of Target: Mast cell carboxypeptidase A
• Uniprot ID: https://www.uniprot.org/uniprot/P15088
• Protein Accession #: NP_001861.2
• Nucleotide Accession #: NM_001870.3
• Protein Size (# AA): 417
• Molecular Weight: 49 KDa