CPA3 ELISA Kit (Human) (OKCD01671)

Catalog No: OKCD01671 (Formerly GWB-KBBFX9)

Size:96 Wells

Human CPA3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	79-99%	93-101%	99-105%	86-94%
  EDTA Plasma (n=5)	84-99%	78-94%	92-99%	91-101%
  Heparin Plasma (n=5)	80-102%	79-98%	79-90%	85-101%

• ELISA Kit Principle: Aviva Systems Biology CPA3 ELISA Kit (Human) (OKCD01671) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CPA3 captured in the well.
• ELISA Kit Range: 7.8 - 500 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-99	96
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma (n=5)	94-102	98

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CPA3 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CPA3 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-CPA3 Microplate: 96 Wells (12 x 8 Well Strips)
  • CPA3 Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated CPA3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 3.0 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase A3, Mast Cell (CPA3). No significant cross-reactivity or interference between Carboxypeptidase A3, Mast Cell (CPA3) and analogues was observed.
• Publications:

  Severe COVID-19 is marked by dysregulated serum levels of carboxypeptidase A3 and serotonin. J Leukoc Biol. (2021) 34057753

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CPA3
• Gene Full Name: Carboxypeptidase A3
• Alias Symbols: MC-CPA
• NCBI Gene Id: 1359
• Protein Name: Mast cell carboxypeptidase A
• Description of Target: This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Expression of this gene may be elevated in human asthma patients.
• Uniprot ID: P15088
• Protein Accession #: NP_001861.2
• Nucleotide Accession #: NM_001870.3
• Protein Size (# AA): 417