Catalog No: OKCD01671 (Formerly GWB-KBBFX9)
Size:96 Wells
Price: $675.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-99%93-101%99-105%86-94%
EDTA Plasma (n=5)84-99%78-94%92-99%91-101%
Heparin Plasma (n=5)80-102%79-98%79-90%85-101%
ELISA Kit PrincipleAviva Systems Biology CPA3 ELISA Kit (Human) (OKCD01671) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CPA3 captured in the well.
ELISA Kit Range7.8 - 500 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9996
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)94-10298
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CPA3 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CPA3 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-CPA3 Microplate96 Wells (12 x 8 Well Strips)
CPA3 Lyophilized Standard2 x 500 pg
100X Biotinylated CPA3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 3.0 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase A3, Mast Cell (CPA3). No significant cross-reactivity or interference between Carboxypeptidase A3, Mast Cell (CPA3) and analogues was observed.

Severe COVID-19 is marked by dysregulated serum levels of carboxypeptidase A3 and serotonin. J Leukoc Biol. (2021) 34057753

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCPA3
Gene Full NameCarboxypeptidase A3
Alias SymbolsMC-CPA
NCBI Gene Id1359
Protein NameMast cell carboxypeptidase A
Description of TargetThis gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Expression of this gene may be elevated in human asthma patients.
Uniprot IDP15088
Protein Accession #NP_001861.2
Nucleotide Accession #NM_001870.3
Protein Size (# AA)417
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