CPA3 ELISA Kit (Human) (OKCD01671)

Catalog No: OKCD01671 (Formerly GWB-KBBFX9)

Size:96WELLS

Human CPA3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	79-99%	93-101%	99-105%	86-94%
  EDTA Plasma (n=5)	84-99%	78-94%	92-99%	91-101%
  Heparin Plasma (n=5)	80-102%	79-98%	79-90%	85-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase A3 (CPA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase A3 (CPA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase A3 (CPA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxypeptidase A3 (CPA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-99	96
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma (n=5)	94-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase A3 (CPA3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase A3 (CPA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CPA3 Microplate: 96 Wells (12 x 8 Well Strips)
  • CPA3 Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated CPA3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 3.0 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase A3, Mast Cell (CPA3). No significant cross-reactivity or interference between Carboxypeptidase A3, Mast Cell (CPA3) and analogues was observed.
• Publications:

  Severe COVID-19 is marked by dysregulated serum levels of carboxypeptidase A3 and serotonin. J Leukoc Biol. (2021) 34057753

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for CPA3 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: CPA3
• Gene Full Name: carboxypeptidase A3
• Alias Symbols: Carboxypeptidase A3;carboxypeptidase A3 (mast cell);mast cell carboxypeptidase A;MC-CPA;tissue carboxypeptidase A.
• NCBI Gene Id: 1359
• Protein Name: Mast cell carboxypeptidase A
• Description of Target: Mast cell carboxypeptidase A
• Uniprot ID: P15088
• Protein Accession #: NP_001861.2
• Nucleotide Accession #: NM_001870.3
• Protein Size (# AA): 417