Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD01262 (Formerly GWB-KBBNF8)
Size:96WELLS
Price: $725.00
SKU
OKCD01262
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for CPA2 ELISA Kit (Rat) (OKCD01262)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)87-99%92-101%98-105%88-97%
EDTA plasma(n=5)96-104%78-95%79-97%78-96%
heparin plasma(n=5)82-93%86-96%90-98%96-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase A2, Pancreatic (CPA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase A2, Pancreatic (CPA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase A2, Pancreatic (CPA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carboxypeptidase A2, Pancreatic (CPA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.781-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-10297
EDTA plasma(n=5)96-104101
heparin plasma(n=5)89-9693
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase A2, Pancreatic (CPA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase A2, Pancreatic (CPA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cpa2 Microplate96 Wells (12 x 8 Well Strips)
Cpa2 Lyophilized Standard2 x 50 ng
100X Biotinylated Cpa2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.31 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase A2, Pancreatic (CPA2). No significant cross-reactivity or interference between Carboxypeptidase A2, Pancreatic (CPA2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCPA2
Gene Full Namecarboxypeptidase A2
Alias SymbolsCPA2(S)
NCBI Gene Id296959
Protein NameCarboxypeptidase A2
Description of TargetCarboxypeptidase A2
Uniprot IDP19222
Protein Accession #NP_001013101
Nucleotide Accession #NM_001013083.2
Protein Size (# AA)417
Write Your Own Review
You're reviewing:CPA2 ELISA Kit (Rat) (OKCD01262)
Your Rating
We found other products you might like!