COX6A2 Antibody (OAGA00659)

Catalog No: OAGA00659

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:Raji
12% SDS PAGE
OAGA00659 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using COX6A2(OAGA00659) antibody at 1:100 dilution.

COX6A2 antibody detects COX6A2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. COX6A2 antibody diluted at 1:500.

COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: COX6A2 protein stained by COX6A2 antibody [N3C3] (OAGA00659) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Monkey
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
  Positive Controls: Raji
  Cellular Localization: Mitochondrion inner membrane
  
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX6A2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.9 mg/ml
• Intended Use: For research use only.
• Application Info: ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
  WB: 1:500~3000
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: COX6A2
• Gene Full Name: Cytochrome c oxidase subunit 6A2, mitochondrial
• Alias Symbols: COX6AH, COXVIAH, MC4DN18
• NCBI Gene Id: 1339
• Protein Name: Cytochrome c oxidase subunit 6A2, mitochondrial
• Description of Target: Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.
• Uniprot ID: Q02221
• Protein Accession #: NP_005196.1
• Molecular Weight: 11 kDa