COX4I1 ELISA Kit (Mouse) (OKCA02366)

Catalog No: OKCA02366

Size:96 Wells

Mouse COX4I1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	107	97-111
  1:2	99	90-102
  1:4	95	84-99
  1:8	105	95-109

• ELISA Kit Principle: Aviva Systems Biology Cox4i1 ELISA Kit (Mouse) (OKCA02366) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cox4i1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cox4i1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cox4i1 captured in the well.
• ELISA Kit Range: 125 - 8,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	108	94-112
  EDTA Plasma (n=4)	107	95-110

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Cox4i1 Microplate: 96 Wells (12 x 8 Well strips)
  • Cox4i1 Lyophilized Standard: 2
  • 100X Biotinylated Cox4i1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 31.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: COX4I1
• Alias Symbols: CO, COX, Cox4, IV-1, COXIV, Cox4a, AL024441, COX IV-1
• NCBI Gene Id: 12857
• Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• Description of Target: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
• Uniprot ID: P19783
• Protein Accession #: NP_001280488
• Nucleotide Accession #: NM_001293559