COX4I1 ELISA Kit (Human) (OKCD08439)

Catalog No: OKCD08439

Size:96WELLS

COX4I1 ELISA Kit (Human) (OKCD08439) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • COX4I1 Microplate: 96 Wells (12 x 8 Well strips)
  • COX4I1 Lyophilized Standard: 2
  • 100X COX4I1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.058ng/mL

Target Info

• Gene Symbol: COX4I1
• Gene Full Name: cytochrome c oxidase subunit 4I1
• Alias Symbols: COX IV-1;COX4;COX4-1;COXIV;COXIV-1;cytochrome c oxidase polypeptide IV;cytochrome c oxidase subunit 4 isoform 1, mitochondrial;cytochrome c oxidase subunit IV;Cytochrome c oxidase subunit IV isoform 1;MC4DN16.
• NCBI Gene Id: 1327
• Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• Description of Target: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
• Uniprot ID: P13073
• Protein Accession #: NP_001305715.1
• Nucleotide Accession #: NM_001318786.1