Catalog No: OKEH07535
Size:96T
Price: $800.00
SKU
OKEH07535
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology COX4I1 ELISA Kit (Bovine) (OKEH07535) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COX4I1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COX4I1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COX4I1 captured in well. | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=7.7% (n = 20) Mean Inter-assay CV%: <=9.4% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. | ||||||||||||||||||||||
:: | Subcellular Location: Mitochondrion inner membrane | ||||||||||||||||||||||
:: | Unigene: Bt.107085 SMR: P00423 String: 9913.ENSBTAP00000049613 | ||||||||||||||||||||||
:: | KEGG: bta:281090 Pfam: PF02936 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Specificity | Natural and recombinant Bovine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | COX4I1 |
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Gene Full Name | cytochrome c oxidase subunit 4I1 |
Alias Symbols | COX IV-1;COX4;cytochrome c oxidase polypeptide IV;cytochrome c oxidase subunit 4 isoform 1, mitochondrial;Cytochrome c oxidase subunit IV isoform 1;cytochrome c oxydase subunit 4. |
NCBI Gene Id | 281090 |
Protein Name | P00423 |
Description of Target | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
Uniprot ID | https://www.uniprot.org/uniprot/P00423 |
Protein Accession # | NP_001001439.1 |
Nucleotide Accession # | NM_001001439.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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