COX1 Antibody - middle region (OAAB06281)

Catalog No: OAAB06281

Size:400ul

All lanes : Anti-COXI Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse spleen lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of COXI Antibody (Center)(Cat#OAAB06281) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IF
  Function: Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
  Cellular Location: Mitochondrion inner membrane; Multi-pass membrane protein.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This COXI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids of human COXI.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine, Chicken, Zebrafish, Mouse, Pig, Rabbit, Rat, Xenopus
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IF: 1:25
  WB: 1:2000

Target Info

• Gene Symbol: COX1
• Alias Symbols: COI, MTCO1
• NCBI Gene Id: 4512
• Protein Name: Cytochrome c oxidase subunit 1
• Uniprot ID: P00395
• Protein Accession #: YP_003024028.1
• Molecular Weight: 57041 Da