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Catalog No: OKEH00627
Size:96T
Price: $525.00
SKU
OKEH00627
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Corticotropin ELISA Kit (Rat) (OKEH00627)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CRH ELISA Kit (Rat) (OKEH00627) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for CRH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRH captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 8.8% (n = 20)
ELISA Kit Component
ComponentAmount
ACTH Microplate96 Wells (12 x 8 Well strips)
ACTH Lyophilized Standard2
100X Biotinylated ACTH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Rat Corticotropin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Alias SymbolsCorticotropin-lipotropin.
Protein NameP01194
Description of TargetPro-opiomelanocortin
Uniprot IDhttps://www.uniprot.org/uniprot/P01194
Protein Accession #NP_112281.1
Nucleotide Accession #NM_001113062.1
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