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Datasheets/Manuals | Printable datasheet for OASE00246 |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Clonality | Monoclonal |
Clone | S60-4 |
Isotype | IgG2b |
Host | Mouse |
Conjugation | Unconjugated |
Application | WB, IHC, ICC, IF, IP |
Additional Information | Background Info: Detects ~180kDa in rat brain membrane preparations |
:: | Scientific Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage. |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of Copper-transporting ATPase1 in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody. |
Reconstitution and Storage | Store at -20C. Shipping with Blue Ice or 4C. |
Immunogen | Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1 |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Specificity | Detects ~180kDa in rat brain membrane preparations. |
Dilution | WB (1:500), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Reference | 1. Samimi G., et al. (2003) Clin. Cancer Res. 9: 5853-9. 2. Samimi G., et al. (2004) Mol Pharmacol. 66: 25-32. 3. Greenough M., et al. (2004) Am. J. Physiol. Cell Physiol. 287: C1463-71. 4. Song, I.S., et al. (2004) Mol. Cancer Ther. 3: 1543-1549. 5. van Dongen, E.M., et al. (2004) Biochem. Biophys. Res. Commun. 323: 789-795. 6. Samimi, G., et al. (2004) Mol Pharmacol 66: 25-32. 7. Morgan, C.T., et al. (2004) J. Biol. Chem. 279: 36363-36371. 8. Barnes, N., et al. (2005) J. Biol. Chem. [Epub]. |
Description | The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage. |
Gene Symbol | ATP7A |
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Gene Full Name | ATPase, Cu++ transporting, alpha polypeptide |
Alias Symbols | ATP7A, ATP 7A, ATPase Cu transporting, DSMAX, FLJ17790, MC1, MC 1, MK, MNK, OHS, Copper pump 1, Menke, OTTHUMP00000062077, SMAX3, ATPase copper transporting alpha polypeptide, ATPase Cu++ transporting alpha polypeptide (Menkes syndrome), Copper transporting ATPase 1, Cu++ transporting P type ATPase, Menkes disease associated protein, Menkes syndrome |
NCBI Gene Id | 538 |
Uniprot ID | Q04656 |
Protein Accession # | NP_000043.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Copper Transporting ATPase 1 Antibody (OASE00246)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "Copper Transporting ATPase 1 Antibody (OASE00246)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Copper Transporting ATPase 1 Antibody (OASE00246)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Copper Transporting ATPase 1 Antibody (OASE00246)"?
This target may also be called "ATP7A, ATP 7A, ATPase Cu transporting, DSMAX, FLJ17790, MC1, MC 1, MK, MNK, OHS, Copper pump 1, Menke, OTTHUMP00000062077, SMAX3, ATPase copper transporting alpha polypeptide, ATPase Cu++ transporting alpha polypeptide (Menkes syndrome), Copper transporting ATPase 1, Cu++ transporting P type ATPase, Menkes disease associated protein, Menkes syndrome" in publications.
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What is the shipping cost for "Copper Transporting ATPase 1 Antibody (OASE00246)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Copper Transporting ATPase 1 Antibody (OASE00246)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Copper Transporting ATPase 1 Antibody (OASE00246)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Copper Transporting ATPase 1 Antibody (OASE00246)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ATP7A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ATP7A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ATP7A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ATP7A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ATP7A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ATP7A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.