Copper Transporting ATPase 1 Antibody : FITC (OASE00246-FITC)

Catalog No: OASE00246-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S60-4
• Isotype: IgG2b
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: IHC, WB, IP
• Additional Information: Background Info: Detects ~180kDa in rat brain membrane preparations
  Scientific Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease,
  characterized by neurological symptoms and hepatic damage.
  
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Copper-transporting ATPase1 in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Samimi G., et al. (2003) Clin. Cancer Res. 9: 5853-9. 2. Samimi G., et al. (2004) Mol Pharmacol. 66: 25-32.
  3. Greenough M., et al. (2004) Am. J. Physiol. Cell Physiol. 287: C1463-71.
  4. Song, I.S., et al. (2004) Mol. Cancer Ther. 3: 1543-1549.
  5. van Dongen, E.M., et al. (2004) Biochem. Biophys. Res.
  Commun. 323: 789-795.
  6. Samimi, G., et al. (2004) Mol Pharmacol 66: 25-32.
  7. Morgan, C.T., et al. (2004) J. Biol. Chem. 279: 36363-36371.
  8. Barnes, N., et al. (2005) J. Biol. Chem. [Epub].
  

Target Info

• Gene Symbol: ATP7A
• Gene Full Name: ATPase, Cu++ transporting, alpha polypeptide
• Alias Symbols: MK, MNK, DSMAX, SMAX3
• NCBI Gene Id: 538
• Uniprot ID: Q04656
• Protein Accession #: NP_000043.3