Catalog No: OCOA00231
Size:20UG
Price: $179.00
SKU
OCOA00231
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COMT Protein Lysate (OCOA00231)

Datasheets/ManualsPrintable datasheet for COMT Protein Lysate (OCOA00231)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCOMT HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCOMT
Gene Full Namecatechol-O-methyltransferase
Alias Symbolscatechol O-methyltransferase;epididymis secretory sperm binding protein Li 98n;HEL-S-98n;testicular tissue protein Li 42.
NCBI Gene Id1312
Protein NameA0A140VJG8, NM_000754, NP_000745, P21964
Description of TargetCatechol O-methyltransferase, catechol O-methyltransferase isoform MB-COMT [Homo sapiens], Homo sapiens catechol-O-methyltransferase (COMT), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P21964
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000745
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000754
Molecular Weight20 kDa
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